SW-403 Cells

SW-403 is a human colorectal adenocarcinoma cell line derived from a poorly differentiated tumor. It has been widely used in research on colorectal cancer, particularly in studies investigating the effects of gastrointestinal hormones on tumor growth. Notably, SW-403 cells have been shown to respond to gastrin and pentagastrin, two gastrointestinal hormones, by increasing their proliferation. These hormones stimulate growth through the gastrin receptor, which is expressed in some colorectal cancers. In contrast, treatment with proglumide, a gastrin receptor antagonist, inhibits the growth of SW-403 cells both in vitro and in vivo, suggesting that gastrin may play a role in promoting tumor growth in this cell line.
In addition to hormone studies, SW-403 cells have been used to investigate the effects of various chemotherapy agents, such as ciprofloxacin, on cancer cell proliferation and apoptosis. Ciprofloxacin has been shown to inhibit DNA synthesis in SW-403 cells and induce apoptosis in a dose-dependent manner. This process involves mitochondrial membrane breakdown, activation of caspases 3, 8, and 9, and upregulation of pro-apoptotic proteins like Bax. The ability of ciprofloxacin to trigger apoptosis in SW-403 cells suggests its potential as an adjunctive therapeutic agent in colorectal cancer treatment.
Overall, SW-403 serves as a useful model for exploring the molecular mechanisms underlying colorectal cancer growth, hormone sensitivity, and chemotherapy-induced apoptosis. Its response to gastrointestinal hormones like gastrin and to chemotherapeutic agents highlights its relevance in both basic cancer biology and drug development research.

Adherent

51 years

Caucasian

1

Supplement the medium with 10% FBS

1 to 2 times per week

Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.

We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.

Yes, in nude mice

Colon antigen 3, positive. The cells are positive for keratin by immunoperoxidase staining. CSAp negative (CSAp-).

SW-403 cells carry a heterozygous Kras mutation in codon12: GGT|BiggerAs|GTT

G6PD, B, PGM1, 1, PGM3, 1-2, 6PGD, A, ES-D, 1, PEP-D, 1