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T47D Cells

T47D Cells

Für die Verwendung dieses Produktes fordert der Hersteller das zu Ihrer Institution passende, ausgefüllte und unterschriebene Formular:

Profit Organisation/Nutzung: CLS_CLEAR.pdf
Non-Profit Organisation/Nutzung: Non_Profit_CLS_Supply_Agreement.pdf

Bitte laden Sie dieses entweder beim Kaufabschluss hoch oder senden es alternativ an die purchasing@hoelzel.de

Item no.	CLS-300353
Manufacturer	CLS Cell Lines Service
Amount	1 cryovial
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Certificate	For a certificate of analysis, please send an inquiry to info@hoelzel.de.
Specific against	Human (Homo sapiens)
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	T-47-D,T47-D,T47D:A,T47D
Available
Manufacturer - Category	Breast cancer cell lines
Description	The T47D cell line, originating from the pleural effusion of an infiltrating ductal carcinoma of the breast, has become a critical resource in breast cancer research. T-47D cells are unique in the realm of cancer research for their hormonal expression profile, particularly for carrying receptors for 17 beta estradiol, various other steroids, and calcitonin. Additionally, T47D cells express the WNT7B oncogene. T47D cells are notable for their progesterone receptor expression not being regulated by estradiol, despite the hormone's abundance within the cells, setting them apart from MCF7 cells, which are widely recognized for their estrogen receptor positivity and are frequently used to explore estrogen's role in tumor proliferation and response to therapies. The utility of the T47D cell line extends to the formation of xenografts in immunodeficient mice, which are valuable for drug testing, observing receptor status changes, and studying angiogenesis. Furthermore, the T-47D cell line is a resource for cancer gene studies, providing insights into the genomic and proteomic landscape that drives breast cancer. By facilitating a deeper understanding of the proteomic and transcriptomic profiles of breast cancer, the t47d breast cancer cell line aids in the identification of new breast cancer cell phenotypes and the development of targeted therapies. T47D cells have been instrumental in studying the effects of hormones like progesterone on breast cancer, offering insights into transcriptional regulation, drug resistance, and the development of xenograft models for therapeutic testing.
Tissue	Breast
Growth properties	Monolayer, adherent
Disease	Invasive ductal carcinoma
Age	54 years
Gender	Female
Ethnicity	Caucasian
Morphology	Epithelial-like
Biosafety Level	1
Culture Medium	RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Medium Supplements	Supplement the medium with 10% FBS
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Fluid Renewal	2 to 3 times per week
Freezing Recovery	After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
Freeze Medium	CM-1 (Cytion catalog number 800100)
Handling of Cryopreserved Cultures	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.
Safety Precautions	When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.
Disclaimer	Our cells are provided for in vitro laboratory research purposes exclusively and are not intended for clinical or diagnostic use, nor are they to be administered to humans or used for veterinary purposes. Users must adhere to all applicable guidelines and regulations for the handling and use of these cells in a research setting.
Warranty	We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.
Amelogenin	X, X
Tumorigenic	Yes, in nude mice
Split Ratio	A ratio of 1:3 to 1:5 is recommended
Seeding Density	1 x 10^4 cells/cm^2
Metastatic Site	Pleural effusion
Mutational Profile	TP53 mut
Karyotype	Mode = 66, dicentric and extra long submetacentric chromosomes
Oncogenes	wnt3 +, wnt7h +, wnt7b+
Receptors Expressed	Estradiol, steroids, calcitonin, androgen, progesterone, glucocorticoid, prolactin, estrogen
Isoenzymes	G6PD, B, PGM1, 1, PGM3, 1, ES-D, 2, Ak-1, 1, GLO-1, 1-2

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cryovial

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Delivery expected until 10/30/2025

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