THP-1 Cells

THP1 cells are a multifacted model with applications in Immune response modeling, Monocyte/macrophage differentiation, Phagocytosis mechanisms, Inflammatory signaling pathways, Drug transport assays

THP1 cells, a spontaneously immortalized monocyte-like cell line derived from the peripheral blood of a 1-year-old monocytic leukemia patient, serve as a critical model in immunological and cancer research. The THP-1 monocyte cell line, known for its ability to differentiate into mature macrophage and dendritic cells, is essential for studying the functions and properties of these immune cells in vitro, including adipose tissue macrophages and M2 mononuclear phagocytes.
THP-1 differentiated macrophages are instrumental in exploring monocyte and macrophage functions, mechanisms, signalling pathways, including cytokine activation and immune modulation, and studying nutrient and drug transport. Further, THP-1 macrophages can be polarized into M1 or M2 macrophages, crucial for studies on immunity and inflammation, innate immunity, and inflammatory responses.
In the context of metabolic and inflammatory diseases, THP-1 cells help explore cytokine profiles, including inflammatory cytokines, and their impact on conditions like human adipocyte apoptosis, illustrating the interplay between inflammation and metabolic health.
Notably, the THP-1 cell line allows for comparative studies with other monocytic leukemia cells and cell lines like U937, facilitating a deeper understanding of monocyte and macrophage biology across different models.
In summary, the THP-1 human monocytic leukemia cell line stands as a valuable tool for a myriad of research avenues, from investigating the intricate mechanisms of the immune system and its role in cancer to understanding the cellular and molecular underpinnings of immune modulation, cytokine activation, and cell proliferation. Its ability to mimic human macrophages and dendritic cells, combined with the ease of manipulation and fast growth rate, cements its status as a widely used cell line in biological and medical research, offering insights into the cellular basis of immunity and inflammation, the response of cancer cells, and the potential for therapeutic intervention.

The monocytic leukemia THP1 cell line grows in suspension and forms clumps due to cells dividing and attaching to the clusters they split from.

Leukemia

Male

1

Supplement the medium with 10% FBS

2 to 3 times per week

Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.

We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.

Lysozyme