Colo-205 Cells

The COLO-205 cell line is a human colorectal adenocarcinoma cell line first established from the metastatic site of the ascites in a 70-year-old Caucasian male. Characterized by its epithelial cell morphology, this cell line is frequently utilized in biomedical research focused on colorectal cancer, particularly in studies related to cancer biology, drug response, and metastatic mechanisms. COLO-205 cells exhibit a hyperdiploid karyotype and are known to form moderately well-differentiated adenocarcinomas when xenografted into immunodeficient mice.

COLO-205 cells express several key oncogenic and tumor suppressor pathways, making them a valuable model for pharmacological testing and cancer research. They are responsive to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) making them suitable for apoptosis studies. Furthermore, these cells have been used extensively to investigate the pharmacodynamics of various chemotherapeutic agents, providing insights into the mechanisms of action and resistance in colorectal cancer therapy. Research utilizing the COLO-205 line has contributed significantly to understanding the biological behaviors typical of colorectal adenocarcinomas, including cellular proliferation, differentiation, and interaction with anticancer drugs.

Adherent/suspension, loosely attached

70 years

Epithelial-like

RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)

Collect suspension cells in a 15 ml tube and carefully rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 10 minutes, then centrifuge the cells growing in suspension and the adherent cells together. Carefully resuspend the cells and dispense into new flasks which contain fresh medium.

After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.

Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.

We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.

Yes, in nude mice

1 x 10^4 cells/cm^2

20 to 25 hours

Negative

Carcinoembryonic antigen (CEA) 1.5 to 4.1 ng/106 cells/10 days, keratin, interleukin 10 (IL-10, interleukin-10)

Stable (MSS)