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KATO-III Cells

KATO-III Cells

Für die Verwendung dieses Produktes fordert der Hersteller das zu Ihrer Institution passende, ausgefüllte und unterschriebene Formular:

Profit Organisation/Nutzung: CLS_CLEAR.pdf
Non-Profit Organisation/Nutzung: Non_Profit_CLS_Supply_Agreement.pdf

Bitte laden Sie dieses entweder beim Kaufabschluss hoch oder senden es alternativ an die purchasing@hoelzel.de

Item no.	CLS-300381
Manufacturer	CLS Cell Lines Service
Amount	1 cryovial
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Certificate	For a certificate of analysis, please send an inquiry to info@hoelzel.de.
Specific against	Human (Homo sapiens)
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	Kato III,Kato-III,KATO III,KATOIII,KatoIII,KATO 3,JTC-28,Japanese Tissue Culture-28
Available
Manufacturer - Category	Gastric cancer cell lines
Description	The KATO-III cell line is a human gastric carcinoma model derived from the metastatic site of a poorly differentiated adenocarcinoma. These cells are widely utilized in research focused on gastric cancer, particularly for studying the molecular mechanisms driving tumor progression, drug resistance, and metastasis. The KATO-III cells exhibit an aneuploid karyotype, characterized by multiple chromosomal abnormalities, which contributes to their aggressive cancer phenotype. They are notably p53 deficient, a feature often associated with increased tumorigenicity and altered responses to chemotherapy, making them a valuable tool for investigating the role of p53 in gastric cancer. KATO-III cells grow in suspension and display a rounded morphology. They possess a high capacity for proliferation, making them suitable for various in vitro applications, including drug screening and cytotoxicity assays. These cells are also used in studies of cell signaling pathways, as their aberrant signaling is a hallmark of gastric cancer pathogenesis. Researchers often utilize KATO-III cells to explore the efficacy of novel therapeutic agents, particularly those targeting HER2, EGFR, and other relevant oncogenic pathways. This cell line is essential for advancing our understanding of gastric cancer biology and for developing targeted therapies aimed at improving patient outcomes.
Tissue	Stomach
Growth properties	Adherent/suspension
Disease	Adenocarcinoma
Age	57 years
Gender	Male
Ethnicity	Asian
Morphology	Spherical
Biosafety Level	1
Culture Medium	Ham's F12, w: 1.0 mM stable Glutamine, w: 1.0 mM Sodium pyruvate, w: 1.1 g/L NaHCO3 (Cytion article number 820600a)
Medium Supplements	Supplement the medium with 10% FBS
Subculturing	Gather the suspension cells in a 15 ml tube and gently wash the adherent cells with PBS lacking calcium and magnesium (use 3-5 ml for T25 flasks and 5-10 ml for T75 flasks). Apply Accutase (1-2 ml for T25 flasks, 2.5 ml for T75 flasks) ensuring full coverage of the cell layer. Allow the cells to incubate at room temperature for 10 minutes. Following incubation, combine and centrifuge both the suspension and adherent cells. After centrifugation, carefully resuspend the cell pellet and transfer the cell suspension into new flasks containing fresh medium.
Fluid Renewal	Every 3 to 5 days
Freezing Recovery	After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
Freeze Medium	CM-1 (Cytion catalog number 800100)
Handling of Cryopreserved Cultures	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.
Safety Precautions	When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.
Disclaimer	Our cells are provided for in vitro laboratory research purposes exclusively and are not intended for clinical or diagnostic use, nor are they to be administered to humans or used for veterinary purposes. Users must adhere to all applicable guidelines and regulations for the handling and use of these cells in a research setting.
Warranty	We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.
Amelogenin	X, X
Tumorigenic	Yes, in cheek pouches of anti thymocyte serum treated hamsters, not tumorigenic in nude mice
Split Ratio	A ratio of 1:2 to 1:8 is recommended
Seeding Density	2 x 10^4 cells/cm^2 will result in a confluent monolayer within 2 to 3 days.
Metastatic Site	Pleural effusion
Doubling Time	36 hours
Antigen Expression	Blood Type B, Rh+
Protein Expression	p53 negative, CEA positive
Karyotype	The stemline chromosome number is hypotetraploid with the 2S component occurring at 6.2%. Nine markers were common to most S metaphases, four markers were less frequent. One (occasionally 2 copies) homogenous staining region (HSR) (t(11, HSR) was present in all metaphases examined, but no double minutes (DM) were detected (Sekiguchi 1978).
Isoenzymes	PGM3, 1, PGM1, 1, ES-D, 1, AK-1, 1, GLO-1, 2, G6PD, B, Phenotype Frequency Product: 0.0742

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cryovial

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Delivery expected until 10/30/2025

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