SK-N-LO Cells

The SK-N-LO cell line is a human neuroblastoma cell line used in research to study neuroblastoma as well as mechanisms of apoptosis and cancer signaling pathways. It is also classified as a primitive neuroectodermal tumor (PNET) cell line and carries the EWS-FLI1 fusion gene, commonly found in Ewing's sarcoma family tumors (ESFT). This fusion gene results from a chromosomal translocation and plays a key role in the oncogenic behavior of these tumor cells.
SK-N-LO cells are particularly sensitive to certain inhibitors targeting oncogenic signaling pathways. For example, the GLI inhibitor GANT61 has been shown to induce caspase-independent apoptosis in SK-N-LO cells. GANT61 disrupts GLI1 and GLI2-mediated transcription in the Hedgehog (Hh) signaling pathway, which is critical for cell survival and proliferation in this cell line. When treated with GANT61, SK-N-LO cells exhibit morphological changes associated with apoptosis, such as chromatin condensation and nuclear fragmentation. Furthermore, GANT61 reduces the expression of proteins like GLI2 and survivin, which are important for cell cycle progression and survival, while increasing the expression of p21, a cyclin-dependent kinase inhibitor.
Additionally, SK-N-LO cells have been utilized to study opioid receptor signaling. These cells have been engineered to express the μ-opioid receptor, making them a valuable model for investigating the interaction between opioid-induced analgesia and intracellular signaling pathways. For instance, studies have shown that morphine stimulates Akt phosphorylation in SK-N-LO cells via the PI3Kγ pathway, a process that can be modulated by cAMP signaling. This highlights the versatility of SK-N-LO cells in exploring both cancer biology and neuropharmacology.

Adherent in collagen-coated flasks

10 years

Caucasian

1

Supplement the medium with 10% FBS

2 to 3 times per week

Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.

We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.

A ratio of 1:6 to 1:12 is recommended

Bone marrow

Deutsch:
Universitäre Kunden: Für den Erwerb ist ein Material Transfer Agreement oder eine Limited Use Label License auszufüllen.
Kommerzielle Kunden: Für den Erwerb ist ein Material Transfer Agreement oder ein Master Supply Agreement auszufüllen.
Nach eingegangener Bestellung werden ihnen alle relevanten Dokumente zugeschickt.

English:
University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase.
Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase.
After the order is received, all relevant documents will be sent to you.