TF-1 Cells

The TF-1 cell line can be applied in various systems due to their responsiveness to multiple cytokines. They provide a good system to investigate the proliferation and differentiation of myeloid progenitor cells. Sensitive to GM-CSF, IL-3, EPO.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately -78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

TF-1 cells are erythroblasts isolated from the bone marrow of a 35-year-old Asian male diagnosed with severe pancytopenia in 1987. These cells are a pivotal model for studying the complex processes of proliferation and differentiation within myeloid progenitor cells. As a cell line, TF-1 is heavily utilized in hematological research to understand the underlying mechanisms that govern cell cycle regulation and development in myeloid lineages.

In addition to their primary role in hematopoietic research, TF-1 cells serve as a robust system for examining the impact of various cytokines on cell survival and growth. Their dependence on specific growth factors such as granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-3 (IL-3) for proliferation makes them an excellent tool for studying cytokine-mediated signaling pathways. This characteristic also makes TF-1 cells useful in evaluating the efficacy of new pharmacological agents that aim to modulate these pathways, thereby contributing significantly to therapeutic advances in treating myeloid disorders and other related diseases.

Suspension

35 years

Japanese

RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)

Initiate cultures with a cell density of 2 x 105 cells/ml and maintain them within the range of 1 x 105 to 1 x 106 cells/ml. For subculturing, transfer the cell suspension to a fresh cell culture flask pre-filled with the correct volume of fresh culture medium.

As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100) (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.

When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.

X, Y

TF-1 cells do not express glycophorin A or carbonyl anhydrase I.

Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.