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MSTO-211H Cells European Partner

Für die Verwendung dieses Produktes fordert der Hersteller das zu Ihrer Institution passende, ausgefüllte und unterschriebene Formular: Bitte laden Sie dieses entweder beim Kaufabschluss hoch oder senden es alternativ an die purchasing@hoelzel.de
Item no. CLS-300450
Manufacturer CLS Cell Lines Service
Amount 1 cryovial
Category
Type Cell line
Certificate For a certificate of analysis, please send an inquiry to info@hoelzel.de.
Specific against Human (Homo sapiens)
Dry ice Yes
ECLASS 10.1 42040401
ECLASS 11.0 42040401
UNSPSC 41106509
Alias MSTO-211 H,MSTO211H,MSTO-211,211H,MeSoTheliOma-211H
Available
Manufacturer - Category
Lung cancer cell lines
Description
The MSTO-211H cell line is derived from a patient with biphasic mesothelioma, specifically from a pleural effusion. It is classified as metastatic, and the patient had not undergone prior radiation or chemotherapy treatments before the establishment of the cell line. MSTO-211H cells are notable for expressing several markers that are significant in understanding both their biological behavior and their potential utility in cancer research. These cells possess high-affinity binding sites for epidermal growth factor (EGF), a property that may contribute to their proliferative capabilities, as EGF is a key regulator of cell growth and differentiation. The presence of EGF receptors suggests these cells could be useful in studying pathways related to growth factor signaling in cancer. In addition to EGF receptors, MSTO-211H cells express neuron-specific enolase (NSE), an enzyme typically found in neurons and neuroendocrine cells. NSE expression in MSTO-211H cells may be indicative of a neuroendocrine differentiation potential, a feature that can be significant for understanding the heterogeneity of mesothelioma tumors. Furthermore, the cells express both the alpha and beta subunits of human chorionic gonadotropin (HCG), a hormone typically produced during pregnancy but also known to be secreted by certain cancers. The expression of HCG subunits in MSTO-211H cells suggests a possible role in tumor biology, potentially related to immune evasion or tumor progression mechanisms. These markers collectively highlight the complex nature of this cell line, making it a valuable model for investigating mesothelioma biology and the effects of therapeutic agents.
Tissue
Lung
Growth properties
Adherent
Disease
Pleural mesothelioma
Age
62 years
Gender
Male
Ethnicity
Caucasian
Biosafety Level
1
Culture Medium
RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Medium Supplements
Supplement the medium with 10% FBS
Subculturing
The cells can reach a saturation density of 400.000 cells per cm2, but will slough off the surface as they attain this density. Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 5 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
Fluid Renewal
2 to 3 times per week
Freezing Recovery
After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
Freeze Medium
Handling of Cryopreserved Cultures

Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Sterility
Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.
To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.
Safety Precautions
When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.
Disclaimer
Our cells are provided for in vitro laboratory research purposes exclusively and are not intended for clinical or diagnostic use, nor are they to be administered to humans or used for veterinary purposes. Users must adhere to all applicable guidelines and regulations for the handling and use of these cells in a research setting.
Warranty
We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.
Amelogenin
X, Y
Tumorigenic
Yes, tumors for med in approximately 20% of nude mice inoculated with MSTO-211H cells
Split Ratio
A ratio of 1:3 to 1:6 is recommended
Seeding Density
1 x 10^4 cells/cm^2
Doubling Time
20 hours
Protein Expression
High affinity binding sites for EGF, expression of Neuron specific enolase (NSE) and alpha and beta subunits of human chorionic gonadotropin (HCG), L-DOPA decarboxylase (DDC), bombesin and neurotensin were not detected.
Karyotype
modal number = 72, range = 70 to 78

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 1 cryovial
Available: In stock
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