HROG05

Manufacturer	CLS Cell Lines Service
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	Human
Amount	1 cryovial
Item no.	CLS-300940
eClass 6.1	32160890
eClass 9.0	32160890
Available
Manufacturers Category	Brain cancer cell lines
Tissue	Brain, L, temporal
Disease	Relapsed Glioblastoma (grade IV)
Age	60 years
Gender	Female
Ethnicity	Caucasian
Morphology	A mixture of fibroblast-likes and epithelial-like cells
Growth Properties	Adherent
Biosafety Level	1
Antigen Expression	HLA-A02+, MHC class I - +MHC class II -, , ICAM-1 + ?-microglobulin +, Beta-2-M+, HLA-E+, HLA-G low, , MIC A low, MIC-B -, GFAP+ , nestin +, vimentin +, S-100+, GBM+, BTSC+
Mutational Profile	IDH 1 2 wt, TP53wt, K-Ras G12D, B-RAFwt , 4q12(PDGFRA) amplified, PTEN P169S/del 212-229
Culture Medium	DMEM:Hams F12
Medium Supplements	10% FBS, 2 mM L-glutamine
Doubling Time	44 to 48 hours
Subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add TrypLE Express (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37 ?C for 10 to 15 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. This cell line will result in single cell suspension.
Split Ratio	A ratio of 1:3 to 1:6 is recommended
Seeding Density	1 x 10^4 cells/cm^2
Fluid Renewal	Every 3 to 5 days
Freeze Medium	CM-1 (Catalog number CLS-800650)
Amelogenin	X, X
NOTE	Deutsch: Universitäre Kunden: Für den Erwerb ist ein Material Transfer Agreement oder eine Limited Use Label License auszufüllen. Kommerzielle Kunden: Für den Erwerb ist ein Material Transfer Agreement oder ein Master Supply Agreement auszufüllen. Nach eingegangener Bestellung werden ihnen alle relevanten Dokumente zugeschickt. English: University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase. Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase. After the order is received, all relevant documents will be sent to you.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

CLS-300940-datasheet.pdf

Request safety datasheet

Simple Order Order Subscription

Amount: 1 cryovial

available

Delivery expected until 6/6/2024