BJAB Cells

Analysis of B cell surface antigens, testing of cytotoxic drugs, mutational analysis, analysis of apoptotic mechanisms, HLA-typing

The BJAB cell line was established in 1973 from a 5-year-old African girl diagnosed with Epstein-Barr virus (EBV)-negative Burkitt’s lymphoma. This specific origin is crucial for research as it provides a distinct model for studying Burkitt’s lymphoma in the absence of EBV influence, which is common in many other lymphoma cell lines. The EBV-negative status of BJAB cells allows researchers to investigate the genetic and environmental factors contributing to lymphomagenesis without the confounding effects of the virus.

BJAB cells are often used in oncological research, especially for exploring the pathophysiology of Burkitt's lymphoma, and for testing therapeutic strategies against it. The cell line displays many of the hallmark features of Burkitt’s lymphoma, including high proliferation rates and a characteristic immunophenotype. Its genetic stability and the robustness with which it can be cultured make it a valuable tool for in vitro experiments aimed at understanding lymphoma biology and assessing the efficacy of anti-cancer drugs.

Suspension

Burkitt lymphoma

Female

Round cells

RPMI 1640, w: 4.5 g/L Glucose, w: 2 mM L-Glutamine, w: 10 mM HEPES, w: 1 mM Sodium pyruvate, w: 1.5 g/L NaHCO3 (Cytion article number 820702a)

Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 2 x 10^5 cells/ml and keep the cell concentration within the range of 1 x 10^5 to 1 x 10^6 cells/ml for optimal growth.

Fast (48 hours)

Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.

We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.

3 x 10^5 cells/ml

46, hypodiploid