« Back
Home /
B-LCL-CDG2

B-LCL-CDG2

Manufacturer	CLS Cell Lines Service
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	Human
Amount	1 cryovial
Item no.	CLS-302013
eClass 6.1	32160890
eClass 9.0	32160890
Available
Manufacturers Category	B lymphoblastoid cell lines \| Rare diseases - cell lines
Tissue	Peripheral blood
Disease	Congenital Disorders of Glycosylation
Applications	Genotyping of CDG effects in immune cells, functional testing (e.g. B cell surface antigens), testing of cytotoxic drugs, mutational analysis, analysis of apoptotic mechanisms, HLA-typing, impact of defective glycosylation of distinct cellular glycoproteins on diverse functions.
Age	Child
Gender	Female
Ethnicity	Caucasian
Morphology	Round cells
Cell Type	B lymphocyte
Growth Properties	Suspension, Cluster
Biosafety Level	2
Surface Antigens	CD60a- (GD3), CD60c-(7-O-acetylated GD3), CD75s+ sialylated lactosaminyl Noligosaccharides), CD77- (Gb3, globotriaosylceramide)
Antigen Expression	CD10-, CD19+, CD20+, CD21+, CD22+, CD23+, CD24+, CD37+m CD38+, CD39+, CD40+, CD53+, CD71+, CD72(+), CD73+, CD74 (+), CD80+, CD81+, CD82+, CD83-, CD84-, CD85+, CD86+, MHC class I+, MHC class II+
Culture Medium	RPMI 1640
Medium Supplements	10% heat-inactivated FBS, 2 mM L-glutamine
Subculturing	Maintain culture between 2-5 x 10^5 cells/ml. Incubate at 5% CO2, 37?C.
Fluid Renewal	Once the medium colour turned into yellow
Freezing Recovery	Medium
Freeze Medium	CM-1 (Catalog number CLS-800100)
Amelogenin	X, X
NOTE	Deutsch: Universitäre Kunden: Für den Erwerb ist ein Material Transfer Agreement oder eine Limited Use Label License auszufüllen. Kommerzielle Kunden: Für den Erwerb ist ein Material Transfer Agreement oder ein Master Supply Agreement auszufüllen. Nach eingegangener Bestellung werden ihnen alle relevanten Dokumente zugeschickt. English: University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase. Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase. After the order is received, all relevant documents will be sent to you.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

CLS-302013-datasheet.pdf

Request safety datasheet

Simple Order Order Subscription

Amount: 1 cryovial

available

Delivery expected until 5/16/2024