Manufacturer |
CLS Cell Lines Service
|
Category |
|
Type |
Cell line |
Specific against |
Human |
Amount |
1 cryovial |
Item no. |
CLS-302018 |
eClass 6.1 |
32160890 |
eClass 9.0 |
32160890 |
Available |
|
Manufacturers Category |
Rare diseases - cell lines |
Tissue |
Peripheral blood |
Disease |
Congenital Disorders of Glycosylation |
Applications |
Genotyping of CDG effects in immune cells, functional testing (e.g. B cell surface antigens), testing of cytotoxic drugs, mutational analysis, analysis of apoptotic mechanisms, HLA-typing, impact of defective glycosylation of distinct cellular glycoproteins on diverse functions. |
Age |
Child |
Gender |
Male |
Ethnicity |
Caucasian |
Morphology |
Round cells |
Cell Type |
B lymphocyte |
Growth Properties |
Suspension, Cluster |
Biosafety Level |
2 |
Surface Antigens |
CD15 (Lewis x)(+), CD15s (sialylated Lewis x)-, CD75s (sialylated lactosaminyl Noligosccharides)+, CD173 (blood group H)-, CD174 (blood group Lewis y)-, CD175 (Tn)-, CD175s (sialylated Tn)-, CD176 (TF)+ |
Antigen Expression |
CD19+, CD20+, CD37+, CD43+, CD44+, CD45+, CD45R0-MHC Class.I+, MHC Class II (HLA-DR)+ |
Culture Medium |
RPMI 1640 |
Medium Supplements |
10% heat-inactivated FBS, 2 mM L-glutamine |
Subculturing |
Maintain culture between 2-5 x 10^5 cells/ml. Incubate at 5% CO2, 37 ?C. |
Fluid Renewal |
Once the medium colour turned into yellow |
Freezing Recovery |
Medium |
Freeze Medium |
|
Amelogenin |
X, Y |
NOTE |
Deutsch:Universitäre Kunden: Für den Erwerb ist ein Material Transfer Agreement oder eine Limited Use Label License auszufüllen. Kommerzielle Kunden: Für den Erwerb ist ein Material Transfer Agreement oder ein Master Supply Agreement auszufüllen.Nach eingegangener Bestellung werden ihnen alle relevanten Dokumente zugeschickt.English:University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase.Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase.After the order is received, all relevant documents will be sent to you. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.