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ASB-XIV

ASB-XIV

Manufacturer	CLS Cell Lines Service
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	Mouse
Amount	1 cryovial
Item no.	CLS-400120
eClass 6.1	32160890
eClass 9.0	32160890
Available
Manufacturers Category	Mouse cell lines
Tissue	Lung
Disease	Pulmonary squamous cell carcinoma
Age	Adult
Gender	Unspecified
Morphology	Epithelial-like
Growth Properties	Adherent
Biosafety Level	1
Tumorigenic	Yes, in Balb/c mouse
Viruses	MAP-test: Negative (Sendai, Ektromelie, Polyoma, K-Virus, Kilham, Reo 3, PVM, LCM, M.pulmonis, MVM, Theilers GD VII, Toolans H-1, MHV, LDV, RCV/SDA, M-Adenovirus, B.piliformis).
Culture Medium	DMEM
Medium Supplements	10% FBS, 2 mM L-glutamine, 4.5 g/L glucose
Doubling Time	70 hours
Subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. Using collagen-coated flasks increases the number of adherent cells.
Split Ratio	A ratio of 1:4 to 1:6 is recommended
Seeding Density	A seeding density of 1 x 10^4 cells/cm^2 is recommended.
Fluid Renewal	Every 3 to 5 days
Freezing Recovery	Allow the cells to adhere for at least 24 to 48 hours.
Freeze Medium	CM-1 (Catalog number CLS-800100)
NOTE	Deutsch: Universitäre Kunden: Für den Erwerb ist ein Material Transfer Agreement oder eine Limited Use Label License auszufüllen. Kommerzielle Kunden: Für den Erwerb ist ein Material Transfer Agreement oder ein Master Supply Agreement auszufüllen. Nach eingegangener Bestellung werden ihnen alle relevanten Dokumente zugeschickt. English: University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase. Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase. After the order is received, all relevant documents will be sent to you.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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CLS-400120-datasheet.pdf

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Amount: 1 cryovial

available

Delivery expected until 5/16/2024