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EL4.IL-2 Cells

EL4.IL-2 Cells

Item no.	CLS-400425
Manufacturer	CLS Cell Lines Service
Amount	1 cryovial
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Certificate	The certificate of analysis can be requested on the website or via email at info@cytion.com. Please indicate the lot number of your product in the email.
Specific against	Mouse (Murine, Mus musculus)
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Available
Manufacturer - Category	Mouse cell lines
Description	The EL4.IL-2 cells, a distinct subline of EL4, which has been derived from the thymus of a mouse that was affected by lymphoma, expresses the unique characteristics of T lymphoblasts. This makes them an exceptional model for exploring the intricacies of T-cell lymphomas and various aspects of the immune system. A key attribute of the EL4.IL-2 cell line is its exceptional capability to produce interleukin-2 (IL-2), an essential cytokine that plays a pivotal role in immune responses. When these cells are exposed to phorbol-12-myristate-13-acetate (PMA), they exhibit a remarkable proficiency in IL-2 production. The levels can reach up to 2500 units/ml after a 24-hour cultivation period. This potent ability to generate high amounts of IL-2 makes EL4.IL-2 cells an ideal candidate for investigating the various roles and functionalities of IL-2 in different biological contexts. The origin of EL4.IL-2 cells from the thymus, a crucial organ for T-cell maturation, confers upon them properties that closely resemble those of lymphoblasts. This aspect, combined with their lymphoma background, provides a unique opportunity to examine the behaviors and characteristics of lymphoblast-like cells in a controlled experimental environment. Moreover, EL4.IL-2 cells are characterized by a moderate doubling time, ranging approximately between 15 to 20 hours. The predictable and consistent growth pattern of these cells is an essential factor that enhances the efficiency and reliability of various research studies.
Tissue	Thymus
Growth properties	Suspension
Disease	Lymphoma
Breed	C57BL/6N
Biosafety Level	1
Viruses	Ectromelia virus (mousepox) negative.
Culture Medium	DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
Medium Supplements	Supplement the medium with 10% horse serum
Subculturing	Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 2 x 10^5 cells/ml and keep the cell concentration within the range of 1 x 10^5 to 1 x 10^6 cells/ml for optimal growth.
Fluid Renewal	2 to 3 times per week
Freezing Recovery	After thawing, allow the cells to recover from the freezing process for at least 24 hours.
Freeze Medium	CM-1 (Cytion catalog number 800100)
Handling of Cryopreserved Cultures	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.
Safety Precautions	When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.
Disclaimer	Our cells are provided for in vitro laboratory research purposes exclusively and are not intended for clinical or diagnostic use, nor are they to be administered to humans or used for veterinary purposes. Users must adhere to all applicable guidelines and regulations for the handling and use of these cells in a research setting.
Warranty	We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.
Split Ratio	A ratio of 1:3 is recommended
Seeding Density	0.1 x 10^6 cells/ml
Products	interleukin-2 (IL-2)
NOTE	Deutsch: Universitäre Kunden: Für den Erwerb ist ein Material Transfer Agreement oder eine Limited Use Label License auszufüllen. Kommerzielle Kunden: Für den Erwerb ist ein Material Transfer Agreement oder ein Master Supply Agreement auszufüllen. Nach eingegangener Bestellung werden ihnen alle relevanten Dokumente zugeschickt. English: University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase. Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase. After the order is received, all relevant documents will be sent to you.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cryovial

available

Delivery expected until 9/4/2025

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