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AH-130 FN

AH-130 FN

Manufacturer	CLS Cell Lines Service
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	Rat
Amount	1 cryovial
Item no.	CLS-500451
eClass 6.1	32160890
eClass 9.0	32160890
Available
Manufacturers Category	Rat cell lines
Tissue	Liver
Disease	Hepatocellular carcinoma
Morphology	Round cells in suspension, Epithelial-like when adherent
Growth Properties	Suspension, few adherent
Biosafety Level	1
Tumorigenic	Yes, in Wistar rats.
Viruses	RAP-test negative. .
Culture Medium	DMEM:Hams F12
Medium Supplements	5% FBS, 2 mM L-glutamine
Subculturing	Resuspend cell suspension in the flask and take representative aliquote to count the cell number per ml. Dilute cell suspension to 1 x 10^5 cells/ml with fresh medium and transfer into new flasks.
Split Ratio	A ratio of 1:2 to 1:4 is recommended
Seeding Density	1 x 10^6 cells/cm^2
Fluid Renewal	Every 3 to 5 days
Freezing Recovery	After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
Freeze Medium	CM-1 (Catalog number CLS-800100)
NOTE	Deutsch: Universitäre Kunden: Für den Erwerb ist ein Material Transfer Agreement oder eine Limited Use Label License auszufüllen. Kommerzielle Kunden: Für den Erwerb ist ein Material Transfer Agreement oder ein Master Supply Agreement auszufüllen. Nach eingegangener Bestellung werden ihnen alle relevanten Dokumente zugeschickt. English: University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase. Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase. After the order is received, all relevant documents will be sent to you.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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CLS-500451-datasheet.pdf

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Amount: 1 cryovial

available

Delivery expected until 5/23/2024