MDBK (NBL-1) Cells

MDBK cells, short for Madin-Darby Bovine Kidney cells (also known as NBL-1), are an exceptional biological resource derived from the kidneys of apparently healthy adult Bos taurus, specifically male individuals. These cells grow adherently and possess an epithelial-like morphology.
One of the remarkable applications of MDBK cells lies in their ability to facilitate in vitro studies on the expression of Eimeria bovis-derived antigens on the host cell surface membrane. Additionally, MDBK cells have been employed in investigations centred around the ubiquitination and degradation of signal transducer and activator of transcription 1 and 2 (STAT1 and STAT2) by the V proteins of paramyxoviruses, such as simian virus five and human parainfluenza virus type 2.
With an average doubling time ranging from 24 to 35 hours, MDBK cells exhibit a moderate proliferation rate. The establishment of the MDBK cell line dates back to February 18, 1957, when S.H. Madin and N.B. Darby successfully derived it from the kidney of a healthy adult steer. Since then, these cells have become a cornerstone in biological research, enabling numerous breakthroughs in various scientific fields.
The karyotype analysis of MDBK cells reveals a modal chromosome number of 51, indicating a hypodiploid state. Within the cell population, the hypodiploid condition manifests as a stemline chromosome number of 2n = 60, with a 2S component occurring in approximately 5% of the cells. Moreover, 11-14 marker chromosomes are typically present, comprising a combination of metacentric, submetacentric, and acro-telocentric chromosomes. Notably, the x chromosome appears monosomic, while no HSR chromosomes or DM's (double minutes) are observed.
MDBK cells exhibit an array of applications in the realm of biological research. Their utility extends to 3D cell culture, enabling scientists to recreate complex tissue-like structures for advanced studies. Furthermore, MDBK cells are invaluable in high-throughput screening, facilitating the rapid and efficient screening of compounds or agents for various purposes. Additionally, these cells play a crucial role in toxicology studies, essential for evaluating the safety and potential adverse effects of substances on living organisms.Regarding viral susceptibility, MDBK cells demonstrate receptiveness to several pathogens, including Vesicular stomatitis Orsay (Indiana) virus, infectious bovine rhinotracheitis virus, bovine rhinotracheitis virus, bovine parvovirus, bovine adenovirus 2 and 3, bovine viral diarrhoea virus 1, and parainfluenza three virus. This susceptibility to a diverse range of viruses makes MDBK cells invaluable for investigating viral pathogenesis and evaluating antiviral strategies.

Monolayer, adherent

Male

Epithelial-like

The line was Tested and shown to be free of bovine diarrhoea virus.

Supplement the medium with 10% FBS

Every 3 days

CM-1 (Cytion catalog number 800100)

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.
To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Our cells are provided for in vitro laboratory research purposes exclusively and are not intended for clinical or diagnostic use, nor are they to be administered to humans or used for veterinary purposes. Users must adhere to all applicable guidelines and regulations for the handling and use of these cells in a research setting.

A ratio of 1:2 to 1:4 is recommended

The cells are susceptibled to bovine diarrhea virus, vesicular stomatitis (Indiana strain), infectious bovine rhinotracheitis virus, bovine parvovirus, bovine adenovirus I and III, and parainfluenza virus 3.

Keratin