V79-4 Cells

The V79-4 cell line is a subline of the original V79 Chinese hamster lung fibroblasts, a lineage that traces back to its development by Ford and Yerganian in 1958 from lung tissue of a young male Chinese hamster. Initially named "Strain V, " the cell line was later renamed "V-79" by Elkind in 1958. In 1966, E.H.Y. Chu, having received the line from W. Sinclair, further isolated the sub-clone V79-4. This detailed lineage highlights the meticulous efforts to create a stable and reliable fibroblast cell model, which has since become a cornerstone in various biological research applications. The V79-4 cell line is particularly renowned for its use in genetic toxicology studies, including assays that investigate mutagenesis, cytotoxicity, and DNA repair mechanisms. One of its key applications is in the study of radiation-induced damage and cellular responses to genotoxic agents, such as in the micronucleus assay. Researchers favor this cell line for its stable karyotype, fibroblast-like morphology, and rapid growth rate, making it an ideal tool for experiments requiring consistent and reproducible results in mammalian cell models. In addition to its role in mutagenesis studies, V79-4 cells are frequently utilized to evaluate the effects of chemical mutagens and pharmaceuticals on cellular integrity. Their robust nature and well-characterized response to genotoxic stress make them highly valuable in studies focused on DNA damage, repair pathways, and the cellular mechanisms involved in maintaining genomic stability. As such, V79-4 cells are a widely trusted model in research fields that include cancer biology, pharmacology, and environmental toxicology.

Monolayer, adherent

Adult

Fibroblast-like

EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c)

Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.

CM-1 (Cytion catalog number 800100)

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.
To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Our cells are provided for in vitro laboratory research purposes exclusively and are not intended for clinical or diagnostic use, nor are they to be administered to humans or used for veterinary purposes. Users must adhere to all applicable guidelines and regulations for the handling and use of these cells in a research setting.

A ratio of 1:6 to 1:14 is recommended

Modal number = 22. Range = 20 to 23 Pseudodiploid. The rate of higher ploidies was 4%. Twelve to marker chromosomes were common to most cells. These include 1p-, 4q+, 4p+, t(6, ?), 7q+ and seven to eight other small markers. Normal N2 and N3 were paired, N1, N5, N6 and N10 were single. Normal X and Y were absent, but a single Xq- was present in every cell.