Disease:
Normal
Species:
Homo sapiens, human Tissue:
Colon Morphology:
Epithelial; although FHC cells exhibit epithelial morphology, cytoplasmic keratins were not detected. Properties:
Adherent Patient:
13 weeks gestation. Medium:
A 1:1 mixture of DMEM:F12 Medium.To make the complete growth medium, add the following components to the base medium: extra 10 mM HEPES (for a final conc. of 25 mM); 10 ng/ml cholera toxin; 0.005 mg/ml insulin; 0.005 mg/ml transferrin; 100 ng/ml hydrocortisone; fetal bovine serum 10% (final conc.) Subculture:
Remove medium, and rinse with 0.05% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Culture at 5% CO2, 37C. A subcultivation ratio of 1:2 to 1:4 is recommended every 10-15 days. Renew medium once every 3 to 4 days depending on cell density. Freezing Medium:
Complete growth medium supplemented with an extra 50% FBS and 10% (v/v) DMSO
Biosafety Level:
I Sterility:
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens:
HIV: Negative Hepatitis B: Negative DNA Profile (STR):
Amelogenin: X, Y CSF1PO: 11, 12 D13S317: 12, 13 D16S539: 9, 11 D5S818:12, 13 D7S820: 8, 12 THO1:6, 9.3 TPOX: 8, 11 vWA: 16 Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: FHC (AddexBio P0009022)