CCD 841 CoN

Disease:

Normal

Origin:

Morphologically the cells resemble epithelial cells; however, the cells do not contain keratin and definitive evidence of epithelial origin is lacking.

Species:

Homo sapiens, human

Tissue:

Colon

Morphology:

Morphologically the cells resemble epithelial cells; however, the cells do not contain keratin and definitive evidence of epithelial origin is lacking.

Properties:

Adherent

Cytogenic data:

The line is diploid and no consistent marker chromosomes were observed.

Patient:

21 weeks gestation fetus

Medium:

AddexBio-formulated EMEM (C0005-01) + 10% FBS

Subculture:

Remove medium, and rinse with 0.05% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Culture at 5% CO2, 37C. A subcultivation ratio of 1:2 to 1:3 is recommended. Renew medium once every 3 to 4 days depending on cell density.

Freezing Medium:

Complete growth medium supplemented with 5% (v/v) DMSO