A9 cells

Disease:

Normal

Origin:

The STO cell line was derived from a continuous line of SIM mouse embryonic fibroblasts. The population which was selected for 6-thioguanine and ouabain resistance is sensitive to HAT medium and is HPRT negative. Used routinely as feeder layers prepared by irradiation or mitomycinc treatment for maintenance of teratocarcinoma stem cells in the undifferentiated state. The cells have been tested and found negative for ectromelia virus (mousepox).

Species:

Mus musculus, mouse

Tissue:

Embryo

Morphology:

Fibroblast

Properties:

Adherent

Cytogenic data:

Not specified

Patient:

Embryo, SIM (Sandos Inbred Mice)

Medium:

AddexBio-formulated DMEM (C0003-01) + 10% FBS

Subculture:

Split sub-confluent cultures (70-80%) at a seed ratio of 1:3 to 1:6, i.e. seeding at 2-4x10, 000 cells/cm^2 is recommended, using 0.25% trypsin; 0.05% EDTA. Culture at 5% CO2, 37C. Renew medium once every 2 to 3 days.

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO