3T3-J2 cells

Disease:

Normal

Origin:

3T3-J2 is a subclone of the original mouse embryonic fibroblast line, 3T3-Swiss Albino. After irradiation, these cells are commonly used as a feeder layer for growth of human keratinocytes.

Species:

Mus musculus, mouse

Tissue:

12-13 day mouse embryos

Morphology:

Fibroblast

Properties:

Adherent

Cytogenic data:

This is a hypertriploid mouse cell line. The modal chromosome number was 68 occurring in 30% of cells. The rate of cells with higher ploidies was 2.4%.

Strain:

This cell line is a subclone of 3T3-Swiss Albino that was established by G. Todaro and H. Green in 1962 from disaggregated Swiss mouse embryos.

Medium:

AddexBio-formulated DMEM (C0003-01) + 10% BCS (bovine calf serum [iron-supplemented; Not heat-inactivated; Not ?-irradiated])

Subculture:

Subculture (60-70% confluency). Do Not allow high confluency. Split the cells at a density of between 3-4 x 103 cells/cm2 using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C.

Freezing Medium:

Complete growth culture medium supplemented with 7.5% (v/v) DMSO