HBEC-5i cells

Disease:

Normal

Origin:

HBEC-5i cells were derived from small fragments of human cerebral cortex obtained from patients who had died of various causes. These brains were devoid of any pathologic abnormalities. Isolation and purification procedures were performed and the cells were cultured. They were then transfected with plasmid containing SV40 large T antigen.

Species:

Homo sapiens, human

Tissue:

Brain, cerebral cortex

Morphology:

Cerebral microvascular endothelium

Properties:

Adherent

Cytogenic data:

HBEC-5i cell line has been shown to retain many of the characteristics of endothelial cells.

Medium:

DMEM/F12 + 10% FBS + 40ug/mL endothelial growth supplement (ECGS)

Subculture:

Note: These cells are cultured on vessels coated with 0.1% Gelatin. Use 1.0 mL of gelatin per 10 cm2 surface area. Incubate at 37C for 45 min. Aspirate gelatin just prior to adding cells to vessels. Subculture sub-confluent cultures (70-80%) at 1:4 to 1:10 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C.

Freezing Medium:

Complete growth culture medium supplemented with 7.5% (v/v) DMSO