MRC-5 cells

Disease:
Normal
Origin:
The MRC-5 cell line was derived from normal lung tissue of a 14-week-old male fetus in 1966.
Species:
Human, Homo sapiens
Tissue:
Lung
Morphology:
Fibroblast
Properties:
Adherent
Cytogenic data:
Chromosome Frequency Distribution 50 Cells: 2n = 46. This is a normal diploid human cell line with 46, XY karyotype. The modal chromosome number was 46, occurring in 70% of cells. The rate of polyploidy was 3.6%. Both X and Y chromosomes were normal. Cytogenetic instability has been reported in the literature for some cell lines.
Patient:
Male, Caucasian, 14 weeks gestation
Medium:
AddexBio-formulated EMEM (C0005-01) + 10% FBS
Subculture:
Split sub-confluent cultures (70-80%) 1:2 to 1:5 using 0.25% trypsin/EDTA. Culture at 5% CO2, 37C. Feed cells once every 2 to 3 days.
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
DNA Profile (STR):
Amelogenin: X, Y
CSF1PO: 11, 12
D13S317: 11, 14
D16S539: 9, 11
D5S818: 11, 12
D7S820: 10, 11
THO1: 8
TPOX: 8
vWA: 15
If use of this culture results in a scientific publication, it should be cited in the publication as: MRC-5 (AddexBio T0016002)
Related cells by Organ: A427 cells
A549 cells
Calu3 cells
H1650 cells
H1703 cells
H1975 cells
H226 cells
H23 cells
H460 cells
H520 cells
HCC4006 cells
SW1573 cells
3D4/2 cells
BEAS-2B cells
MRC-5 cells
Mv 1 Lu cells
RLE-6TN cells

Cell Lines