Mv1Lu cells

Disease:
Normal
Origin:
The Mv 1 Lu cell line was derived from trypsinised lungs of several nearly full-term, unsexed fetuses of the Aleutian mink. Used for focus forming assays for murine and feline sarcoma viruses.
Species:
Neovison vison, American Mink
Tissue:
Lung
Morphology:
Epithelial
Properties:
Adherent
Cytogenic data:
Both male and female diploid cells as well as pseudodiploid cells are present. Approximately 58% of the cells have a chromosome number within + or - 1 of the diploid and one dicentric chromosome is present in some cells of the population. Both male and female diploid cells as well as pseudodiploid cells are present. Approximately 58% of the cells have a chromosome number within + or - 1 of the diploid and one dicentric chromosome is present in some cells of the population.
Patient:
Male and female mixed, near term fetus
Medium:
AddexBio-formulated EMEM (C0005-01) + 10% FBS
Subculture:
Split sub-confluent cultures (70-80%) 1:3 to 1:4 i.e. seeding at 2-4x10, 000 cells/cm^2 using 0.25% trypsin; 0.05% EDTA. Culture at 5% CO2, 37C. Renew medium once every 2 to 3 days.
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: Mv 1 Lu (AddexBio T0016003)
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Cell Lines