Mv1Lu cells

Disease:

Normal

Origin:

The Mv 1 Lu cell line was derived from trypsinised lungs of several nearly full-term, unsexed fetuses of the Aleutian mink. Used for focus forming assays for murine and feline sarcoma viruses.

Species:

Neovison vison, American Mink

Tissue:

Lung

Morphology:

Epithelial

Properties:

Adherent

Cytogenic data:

Both male and female diploid cells as well as pseudodiploid cells are present. Approximately 58% of the cells have a chromosome number within + or - 1 of the diploid and one dicentric chromosome is present in some cells of the population. Both male and female diploid cells as well as pseudodiploid cells are present. Approximately 58% of the cells have a chromosome number within + or - 1 of the diploid and one dicentric chromosome is present in some cells of the population.

Patient:

Male and female mixed, near term fetus

Medium:

AddexBio-formulated EMEM (C0005-01) + 10% FBS

Subculture:

Split sub-confluent cultures (70-80%) 1:3 to 1:4 i.e. seeding at 2-4x10, 000 cells/cm^2 using 0.25% trypsin; 0.05% EDTA. Culture at 5% CO2, 37C. Renew medium once every 2 to 3 days.

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO