WI-38 cells

Disease:

Normal

Origin:

The WI-38 human diploid cell line was derived by Leonard Hayflick from normal embryonic (3 months gestation) lung tissue.

Species:

Homo sapiens, human

Tissue:

Lung

Morphology:

Fibroblast

Properties:

Adherent

Cytogenic data:

Normal diploid

Patient:

Female, Caucasian, 3 months gestation fetus

Medium:

AddexBio-formulated EMEM (C0005-01) + 10% FBS

Subculture:

Subculture sub-confluent cultures (70-80%) at 1:2 to 1:4 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C.

Freezing Medium:

Complete growth culture medium supplemented with 7.5% (v/v) DMSO