WI-38 cells

Disease:
Normal
Origin:
The WI-38 human diploid cell line was derived by Leonard Hayflick from normal embryonic (3 months gestation) lung tissue.
Species:
Homo sapiens, human
Tissue:
Lung
Morphology:
Fibroblast
Properties:
Adherent
Cytogenic data:
Normal diploid
Patient:
Female, Caucasian, 3 months gestation fetus
Medium:
AddexBio-formulated EMEM (C0005-01) + 10% FBS
Subculture:
Subculture sub-confluent cultures (70-80%) at 1:2 to 1:4 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C.
Freezing Medium:
Complete growth culture medium supplemented with 7.5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
DNA Profile (STR):
Amelogenin: X
CSF1PO: 10, 12
D13S317: 11
D16S539: 11, 12
D5S818: 10
D7S820: 9, 11
THO1:8, 9.3
TPOX: 8
vWA: 19, 20
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: WI-38 (AddexBio T0016037)

Cell Lines