D283Med Cells

3D cell culture, Neuroscience

The D283Med cell line is a human medulloblastoma cell line that was derived from the cerebellum of a 6-year-old male. Medulloblastoma is a type of primitive neuroectodermal tumor that primarily affects children and is located in the cerebellum, the part of the brain responsible for motor control and coordination. D283Med cells are widely used in oncological research, particularly in studies focused on the biology and pharmacology of medulloblastomas.

This cell line exhibits an adherent growth pattern and has been used extensively to explore the molecular pathways involved in medulloblastoma pathogenesis, such as the Sonic Hedgehog (SHH) and WNT signaling pathways, which are known to play significant roles in the development and progression of these tumors. Researchers utilize the D283Med line to assess therapeutic efficacy and resistance, study gene expression profiles, and explore novel therapeutic targets. The line's robust growth and typical medulloblastoma genetic features make it a valuable model for preclinical studies aimed at understanding tumor biology and testing anticancer drugs.

Furthermore, D283Med cells are utilized in genetic studies to understand the impact of mutations and to assess mechanisms of metastasis and recurrence in medulloblastoma. They provide a crucial tool for the investigation of oncogenic processes at the cellular level, thereby contributing significantly to the development of targeted therapies for this aggressive pediatric brain tumor.

Clusters in Suspension/Adherent

6 years

European

1

Supplement the medium with 10% FBS

CM-1 (Cytion catalog number 800100)

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.
To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Our cells are provided for in vitro laboratory research purposes exclusively and are not intended for clinical or diagnostic use, nor are they to be administered to humans or used for veterinary purposes. Users must adhere to all applicable guidelines and regulations for the handling and use of these cells in a research setting.

Yes, in nude mice

The karyotype is 45, XY, -7, -8, -17, -20, der(20)t(1, 20)(q12, q13), 8q+, 17p+ (range = 41 to 46). This is a hypodiploid cell line with a frequency of higher ploidies of 5.4%. Three marker chromosomes are present in all cells. They are: der(20)t(1, 20)(q12, q13), 8q+ and 17p+. N7, N17 and N20 have single copies. The single X is structurally normal, and the Y chromosome is present as confirmed by fluorescence microscopy.