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GH3 Cells

GH3 Cells

Für die Verwendung dieses Produktes fordert der Hersteller das zu Ihrer Institution passende, ausgefüllte und unterschriebene Formular:

Profit Organisation/Nutzung: CLS_CLEAR.pdf
Non-Profit Organisation/Nutzung: Non_Profit_CLS_Supply_Agreement.pdf

Bitte laden Sie dieses entweder beim Kaufabschluss hoch oder senden es alternativ an die purchasing@hoelzel.de

Item no.	CLS-300383
Manufacturer	CLS Cell Lines Service
Amount	1 cryovial
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Certificate	For a certificate of analysis, please send an inquiry to info@hoelzel.de.
Specific against	Rat (Rattus norvegicus)
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	GH 3
Available
Manufacturer - Category	Rat cell lines
Description	The GH3 cell line, originating from a rat pituitary tumor, is a critical resource in the study of pituitary functions, particularly regarding the secretion of prolactin and growth hormone. These cells possess characteristics of both somatotropic and lactotropic cells, enabling detailed investigations into pituitary hormones and their regulatory mechanisms. The cell line is extensively utilized to understand the effects of hormonal treatments and genetic modifications on the secretion of these hormones. GH3 cells respond significantly to thyroid-stimulating hormones, making them a valuable model for assays that measure the impact of various compounds on pituitary gland activities. Research employing GH3 cells often delves into how these cells react to different hormonal stimuli. For example, hydrocortisone is known to promote growth hormone production while inhibiting prolactin output in these cells, making GH3 a preferred model for exploring hormonal balance and the endocrine system’s response to stress and other physiological factors. Such studies are pivotal in advancing our understanding of pituitary gland disorders and crafting therapies for conditions like growth deficiencies and hyperprolactinemia. Moreover, GH3 cells are instrumental in pharmacological testing and biotechnological applications aimed at developing treatments for pituitary-related disorders. Their ability to produce more growth hormone compared to GH1 cells, along with prolactin, allows researchers to examine the regulation and effects of these hormones under various conditions. This unique profile is essential for understanding the complex interactions within the endocrine system and for the development of targeted therapeutic interventions.
Tissue	Brain, Pituitary gland
Growth properties	Adherent, clusters in suspension
Disease	Neoplasm
Age	7 months
Gender	Female
Breed	Wistar Furth
Morphology	Epithelial-like
Biosafety Level	1
Culture Medium	Ham's F12, w: 1.0 mM stable Glutamine, w: 1.0 mM Sodium pyruvate, w: 1.1 g/L NaHCO3 (Cytion article number 820600a)
Medium Supplements	Supplement the medium with 15% horse serum, 2.5% heat-inactivated FBS
Subculturing	Gather the suspension cells in a 15 ml tube and gently wash the adherent cells with PBS lacking calcium and magnesium (use 3-5 ml for T25 flasks and 5-10 ml for T75 flasks). Apply Accutase (1-2 ml for T25 flasks, 2.5 ml for T75 flasks) ensuring full coverage of the cell layer. Allow the cells to incubate at room temperature for 10 minutes. Following incubation, combine and centrifuge both the suspension and adherent cells. After centrifugation, carefully resuspend the cell pellet and transfer the cell suspension into new flasks containing fresh medium.
Freeze Medium	CM-1 (Cytion catalog number 800100)
Handling of Cryopreserved Cultures	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.
Safety Precautions	When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.
Disclaimer	Our cells are provided for in vitro laboratory research purposes exclusively and are not intended for clinical or diagnostic use, nor are they to be administered to humans or used for veterinary purposes. Users must adhere to all applicable guidelines and regulations for the handling and use of these cells in a research setting.
Warranty	We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.
Products	growth hormone, prolactin

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cryovial

available

Delivery expected until 10/30/2025

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