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SK-MEL-2

SK-MEL-2

Manufacturer	CLS Cell Lines Service
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	Human
Amount	1 cryovial
Item no.	CLS-300423
eClass 6.1	32160890
eClass 9.0	32160890
Available
Manufacturers Category	Skin cancer cell lines
Tissue	Skin
Disease	Melanoma
Metastatic Site	Skin of thigh
Age	60 years
Gender	Male
Ethnicity	Caucasian
Morphology	Polygonal
Growth Properties	Adherent
Biosafety Level	1
Isoenzymes	PGM3, 1, PGM1, 1, ES-D, 1, AK-1, 1, GLO-1, 2, G6PD, B
Tumorigenic	Yes, in nude mice. Forms malignant melanoma
Karyotype	(P6) hypodiploid to hypertetraploid with abnormalities including dicentrics, secondary constrictions and large telocentric marker. Phenotype Frequency Product: 0.0742
Culture Medium	DMEM
Medium Supplements	10% FBS, 2 mM L-glutamine, 4.5 g/L glucose
Subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
Split Ratio	A ratio of 1:3 to 1:6 is recommended
Seeding Density	1 x 10^4 cells/cm^2
Fluid Renewal	2 to 3 times per week
Freeze Medium	CM-1 (Catalog number CLS-800100)
Amelogenin	X, X

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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CLS-300423-datasheet.pdf

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Amount: 1 cryovial

available

Delivery expected until 5/23/2024