V79 Cells

V79 cells are a widely used and established cell line in biological research, particularly in the study of DNA repair and DNA damage. These cells have a shortened cell cycle, are readily mutagenized to make stable mutant lines deficient in DNA repair enzymes and related DNA damage response functions, and are particularly useful for gene toxicity assays due to their stability of karyotype and morphology. V79 cells have been widely utilized in studies on x-ray, UV radiation, and oxidizing agent-induced DNA damage and repair, as well as investigations into cellular signaling pathways, apoptosis, inflammation, and the effects of various chemicals and compounds on cellular growth and viability. Their extensive use in research attests to their usefulness and importance in biological science.

V79 cells are a Chinese hamster lung fibroblast cell line, commonly used in genetic, toxicological, and radiobiological research. They originate from the lung tissue of the Chinese hamster and are particularly valued for their rapid growth rate and stable karyotype, making them a reliable model for various laboratory studies.
One of the primary uses of V79 cells is in cytotoxicity and genotoxicity testing. These cells are employed to assess the potential DNA-damaging effects of chemical compounds and radiation, providing crucial data for risk assessment and safety evaluations. V79 cells are highly responsive to mutagens and carcinogens, making them an excellent choice for mutagenicity assays, such as the micronucleus test and chromosome aberration test.
In radiation biology, V79 cells are used to study the effects of ionizing radiation on cellular structures and to evaluate the efficacy of radioprotective substances. Their sensitivity to radiation-induced damage allows researchers to investigate the mechanisms of DNA repair, cell cycle arrest, and apoptosis following exposure to various types of radiation.
V79 cells are also instrumental in pharmacological research, particularly in drug screening processes where their robust growth and reproducibility are advantageous for high-throughput assays. They are used to test the cytotoxic effects of new drugs and to study the cellular uptake and metabolism of pharmaceutical compounds.
Overall, the V79 cell line is a versatile tool in biomedical research, contributing to our understanding of cellular responses to environmental agents and aiding in the development of safer and more effective therapeutic interventions.

Adherent

Fibroblast

DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)

Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.

CM-1 (Cytion catalog number 800100)

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.
To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Our cells are provided for in vitro laboratory research purposes exclusively and are not intended for clinical or diagnostic use, nor are they to be administered to humans or used for veterinary purposes. Users must adhere to all applicable guidelines and regulations for the handling and use of these cells in a research setting.

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