CHL Cells

The CHL (Chinese Hamster Lung) cell line is derived from the lung tissue of the Chinese hamster, Cricetulus griseus. This cell line is commonly used in biomedical research due to its sensitivity to mutagens and its utility in cytogenetic tests such as the in vitro chromosomal aberration assay. The CHL cell line has proven especially useful in genetic toxicology for evaluating the potential genotoxicity of chemical compounds. Its genomic stability and relatively high proliferation rate make it a suitable model for studying mechanisms of mutation and for assessing the cytotoxicity of various substances.

CHL cells grow in a monolayer and are adherent, with a fibroblast-like morphology. They are karyotypically male and have been used extensively in research that requires a mammalian system for metabolic activation of chemical compounds. The cell line supports the growth of various viruses and is hence also used in virology research. It's important to maintain them under carefully controlled conditions to prevent changes in their characteristics and to ensure reproducibility of experimental results. The CHL cell line continues to be a critical resource in the fields of toxicology, pharmacology, and molecular biology.

Adherent

1

Supplement the medium with 10% FBS

2 to 3 times per week

Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.

We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.

Human Tissue Plasminogen Activator(T-Pa)