F81 Cells

F81 cells are a feline cell line, specifically derived from feline kidney tissue. These cells are primarily used in research related to the study of feline viruses, including the feline calicivirus (FCV), which is a significant pathogen affecting cats globally. The F81 cell line is noted for its susceptibility to various strains of FCV, making it an indispensable tool in virology research for the development of vaccines and therapeutic strategies aimed at controlling viral infections in felines.

In addition to their use in studying FCV, F81 cells are also utilized in research into other viral pathogens affecting cats, such as feline herpesvirus and feline immunodeficiency virus. Their robustness and ease of culture make F81 cells a valuable model for cellular and molecular biology studies, facilitating advancements in understanding the cellular mechanisms of viral replication and host-pathogen interactions. These cells contribute significantly to translational research, potentially leading to improved diagnostic methods and treatments for feline diseases.

Adherent

Female

1

Supplement the medium with 10% FBS

2 to 3 times per week

Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.

We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.