Hey Cells

HEY Cells, derived from a human ovarian cancer xenograft, are a valuable resource for cancer researchers seeking to advance their understanding of papillary cystadenocarcinoma, a moderately differentiated form of ovarian cancer. The parental cell line, HEY, was initially obtained from a peritoneal sample of a Caucasian patient diagnosed with this specific type of cancer.
A derivative known as HEY-T30 (CRL-3252) was developed through a carefully designed process to enhance the versatility and usefulness of HEY Cells. This involved subjecting the parental HEY cell line to stepwise escalating concentrations of Taxol for six months. HEY-T30 is now maintained in a medium containing 30 nmol/L Taxol, ensuring its stability and consistency. It is worth noting that Jurriaan Brouwer-Visser Institution is the designated repository for this cell line.These epithelial-like cells closely resemble human cells, making them an excellent model for studying ovarian cancer. HEY, Cells exhibit a rapid doubling time of approximately 30 hours, allowing for efficient and time-effective experimentation. Researchers can utilize these cells to investigate various aspects of cancer biology, such as tumour formation, metastasis, and drug response.
HEY, Cells are particularly well-suited for applications involving 3D cell culture, a technique that more closely mimics the physiological environment of tumours. Their ability to grow in semisolid culture and as xenografts in immunologically deprived CBA/CJ mice highlights their adaptability and potential for in vivo studies. By incorporating HEY Cells into cancer research, scientists can uncover crucial insights into the development and progression of papillary cystadenocarcinoma. These cells are invaluable for exploring novel therapeutic strategies, identifying potential drug targets, and evaluating treatment efficacy.
In summary, HEY Cells provide researchers with a robust and reliable resource for investigating ovarian cancer. With their origins in a patient sample and their epithelial-like morphology, these cells faithfully replicate key characteristics of papillary cystadenocarcinoma. Their applications in 3D cell culture and cancer research make them essential in advancing our understanding of this challenging disease.

Adherent

Unspecified

European

DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)

Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.

Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.

We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.

Yes

20 to 30 hours