HK-2 Cells

The HK-2 cell line is a well-characterized human proximal tubular epithelial cell line derived from normal adult kidney tissue. These cells exhibit typical epithelial morphology and retain many of the biochemical and functional properties of proximal tubular cells, making them a valuable model for studying renal physiology and pathophysiology. HK-2 cells are known for their ability to perform active transport and exhibit brush border enzyme activities, which are essential for their role in renal reabsorption processes.
HK-2 cells express a range of transporters and receptors, including those for glucose, amino acids, and various ions, reflecting their role in renal filtration and reabsorption. They are also responsive to hormonal regulation, such as by parathyroid hormone and aldosterone, which influence their transport activities. Due to these characteristics, HK-2 cells are extensively used in nephrotoxicity studies, drug screening, and research on renal diseases such as acute kidney injury and chronic kidney disease.
Moreover, HK-2 cells have been utilized in studies investigating renal cell carcinoma and other kidney-related cancers. They provide a reliable in vitro system for examining cellular responses to toxic agents, oxidative stress, and hypoxia. Researchers also employ HK-2 cells to explore the molecular mechanisms underlying fibrosis and inflammation in the kidney. Overall, the HK-2 cell line is a critical tool in renal research, offering insights into both normal kidney function and disease pathogenesis.

Adherent

Male

Epithelial

EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c)

Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.

CM-1 (Cytion catalog number 800100)

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.
To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Our cells are provided for in vitro laboratory research purposes exclusively and are not intended for clinical or diagnostic use, nor are they to be administered to humans or used for veterinary purposes. Users must adhere to all applicable guidelines and regulations for the handling and use of these cells in a research setting.

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Alkaline Phosphatase, Gamma Glutamyltranspeptidase, Leucine Aminopeptidase, Acid Phosphatase, Cytokeratin, Alpha 3, Beta 1 Integrin, Fibronectin