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ARPE-19

ARPE-19

Manufacturer	CLS Cell Lines Service
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	Human
Amount	1 cryovial
Item no.	CLS-305025
eClass 6.1	32160890
eClass 9.0	32160890
Available
Manufacturers Category	Skin cancer cell lines
Tissue	Eye, retinal pigmented epithelium, retina
Age	19 years
Gender	Male
Morphology	Epithelial
Growth Properties	Adherent
Biosafety Level	1
Protein Expression	Rpe-Specific Markers Cralbp And Rpe-65
Antigen Expression	RPE-specific markers CRALBP and RPE-65
Tumorigenic	Yes
Culture Medium	DMEM:Hams F12
Medium Supplements	10% FBS
Subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
Split Ratio	1:3 to 1:5
Fluid Renewal	2 to 3 times per week
Freeze Medium	CM-1 (Catalog number CLS-800100)
Amelogenin	X, Y

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cryovial

available

Delivery expected until 5/16/2024