KLE Cells

The KLE cell line is an adherent cell line derived from the endometrium of a White, female patient with adenocarcinoma. This cell line was established from a 64-day-old patient and has since become a vital tool in endometrial cancer research. KLE cells were deposited by GR Richardson and are known for their tumorigenic properties, as they form tumors within 21 days with 100% frequency when inoculated subcutaneously in nude mice. These tumors do not form glands but exhibit microvilli, junctional complexes, and nucleolar channel systems similar to those found in normal endometrium under progestational stimulation.

KLE cells express blood type O and are Rh-positive, which can be relevant for specific studies involving antigen expression. The cell line is commonly used to study the pathophysiology of endometrial carcinoma, with particular interest in its estrogen receptor-negative and progesterone receptor-positive status. This receptor profile makes KLE cells highly suitable for research into progesterone's role in endometrial cancer progression. Electron microscopy studies of KLE cell-derived tumors have provided detailed insights into the cellular ultrastructure, making this cell line an essential resource for understanding the morphological aspects of endometrial adenocarcinoma.

Adherent

64 years

European

1

Supplement the medium with 10% FBS

2 times per week

Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.

We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.

Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1?10^7 cells.

114 hours