J82 Cells

The J82 cell line is derived from a human bladder transitional cell carcinoma, offering a robust in vitro model for studying urothelial cancer. These cells exhibit epithelial morphology and are adherent in culture, making them suitable for a variety of experimental applications, including cancer biology research, drug screening, and molecular analysis. J82 cells are known to express markers characteristic of bladder carcinoma, including cytokeratins, which are valuable for understanding the molecular pathways involved in bladder cancer progression and for identifying potential therapeutic targets.

The J82 cell line is particularly useful for studies focusing on the mechanisms of drug resistance, metastasis, and the role of genetic mutations in bladder cancer. Researchers have utilized this cell line to explore the effects of chemotherapeutic agents and to identify novel compounds that may inhibit cancer cell growth. Additionally, J82 cells are frequently used in gene expression studies to investigate the regulation of oncogenes and tumor suppressor genes within the context of bladder cancer. As with all cancer cell lines, J82 should be handled under strict laboratory conditions, ensuring its use is restricted to research applications and not for any therapeutic or in vivo purposes.

Adherent

58 years

European

1

Supplement the medium with 10% FBS

2 to 3 times per week

Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.

We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.

Yes

HLA A2, Aw32, B5, B12, Cw5, Blood Type A