6T-CEM Cells

The 6T-CEM cell line is a mutant derivative of the human acute lymphoblastic leukemia (ALL) T-cell line CCRF-CEM. It was developed by exposing the parent CEM cells to 6-thioguanine, leading to the selection of a subline that exhibits resistance to this compound. This resistance is a result of the inactivation of the HPRT gene, which is critical in the purine salvage pathway. The 6T-CEM cells have been particularly valuable in studying drug resistance mechanisms, especially concerning purine analogs like 6-thioguanine. Additionally, these cells are characterized by their secretion of a unique T-cell suppressor inducer factor (SIF), which is not only non-mitogenic and non-cytotoxic but also capable of suppressing T-cell proliferation while sparing B-cell proliferation at certain dilutions. 6T-CEM cells and their subclones, like 6T-CEM-20, have shown a significant increase in the production of this suppressor-inducer factor, which has potential applications in immunological research, particularly in the study of T-cell regulation and immune suppression. The SIF secreted by these cells has been shown to suppress up to 90% of mitogen-induced T-cell proliferation at extremely high dilutions (up to 10^-9), making these cells a potent model for exploring therapeutic strategies that involve modulation of the immune response. The use of these cells in various experimental setups has provided insights into the molecular underpinnings of immune suppression, with potential implications for the development of treatments for autoimmune diseases and in the context of organ transplantation to prevent graft rejection.

Suspension

4 years

Asian

2

Supplement the medium with 10% FBS

2 to 3 times per week

Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.

We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.

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