HAL-01 Cells

The HAL-01 cell line is derived from the peripheral blood of a female adolescent with a diagnosis of acute lymphoblastic leukemia (ALL), specifically the L2 subtype. This cell line is particularly notable for containing the t(17; 19)(q22; p13) chromosomal translocation, which results in the TCF3-HLF (E2A-HLF) fusion gene. This genetic feature is critical in the study of leukemia as it influences the behavior of leukemia cells, including aspects of their growth, differentiation, and response to therapies.

The presence of the TCF3-HLF fusion gene in the HAL-01 cell line makes it an invaluable resource for oncological research, particularly for studies focused on the mechanisms of leukemogenesis and the development of targeted therapies for leukemia. The fusion protein encoded by this gene is involved in the regulation of gene transcription and has been associated with poor prognosis in patients, underscoring the importance of this cell line in therapeutic development and prognostic research in acute lymphoblastic leukemia.

Suspension

Female

1

Supplement the medium with 10% FBS

2 to 3 times per week

Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.

We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.

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