AtT-20 Cells

The AtT-20 cell line is a well-characterized mouse pituitary tumor cell line derived from anterior pituitary cells. These cells originate from a strain of mice known as AtT-20/D16v-F2, and are primarily used for the study of pituitary function and regulation, especially focusing on the synthesis and secretion of adrenocorticotropic hormone (ACTH). ACTH is crucial for adrenal gland function and is a key player in the stress response and metabolic regulation.

AtT-20 cells exhibit typical features significant for studies in neuroendocrinology and pharmacology, such as the production and secretion of pro-opiomelanocortin (POMC), the precursor molecule for ACTH. The cells are responsive to corticotropin-releasing hormone (CRH) and other hypothalamic hormones, making them an excellent model for exploring the hypothalamic-pituitary-adrenal (HPA) axis in vitro. Moreover, AtT-20 cells can be used to investigate the mechanisms of peptide hormone processing, packaging, and secretion, given their well-defined secretory pathways.

In terms of applications, AtT-20 cells have been utilized in various studies including those focusing on gene expression profiles under different treatment conditions, intracellular signaling pathways involving cAMP, and the effects of genetic modifications on hormone secretion. These cells are also valuable in the assessment of the pharmacological properties of potential drug candidates targeting HPA axis components.

Adherent/suspension

LAF1

1

Supplement the medium with 2.5% FBS, 15% horse serum

2 to 3 times per week

Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.

We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.

Adrenocorticotropic Hormone(Acth)