S-180

Item no.	CLS-305165
Manufacturer	CLS Cell Lines Service
Amount	1 cryovial
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	Mouse (Murine, Mus musculus)
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	CCRF S-180 II, CCRF S 180 II, S-180 II, CCRF S-180, S180, Sarcoma-180, Sarcoma 180, CCRF-180II
Available
Manufacturer - Category	Mouse cell lines
Tissue	Sarcoma
Growth properties	Mixed, Adherent And Suspension
Disease	Mouse fibrosarcoma
Breed	Swiss Webster
Morphology	Rounded
Biosafety Level	1
Culture Medium	EMEM
Medium Supplements	10% FBS, 2 mM L-glutamine, 1% NEAA
Subculturing	Remove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of Accutase solution to flask and observe cells under a microscope until the cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37?C.
Fluid Renewal	2 to 3 times per week
Freeze Medium	CM-1 (Catalog number CLS-800100)
Split Ratio	1:2 to 1:4
Metastatic Site	Ascites

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cryovial

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Delivery expected until 5/29/2025

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