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NCH612 growing culture

NCH612 growing culture

Item no.	CLS-330121
Manufacturer	CLS Cell Lines Service
Amount	1 cell culture flask
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	other
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Available
Growth properties	Spheroid culture
Age	39 years
Gender	Male
Ethnicity	Caucasian
Biosafety Level	1
Culture Medium	GBM-MG
Subculturing	Mechanical dissociation by pipetting (5-10 times) with an Eppendorf pipette and 1000 Î¼l filter tips after centrifugation of the cells (5min, 300g, RT).
Fluid Renewal	Fresh medium must be added every 2-3 days (2-5 ml depending on the size of the cell culture flask).
Freezing Recovery	Slow. After thawing allow the cells to recover from the freezing process for at least 48 hours.
Freeze Medium	CM-ACF (CLS catalog number 800650)
Amelogenin	X, X
Split Ratio	A ratio of 1:2 to 1:5 is recommended
Seeding Density	1 x 10^5 cells/mL
Manufacturer - Citation	NCH612 (CLS catalog number 300121)
CSF1PO	11, 12
D13S317	10
D16S539	11, 13
D5S818	11, 13
D7S820	10, 11
TH01	6, 7
TPOX	8, 12
vWA	17
D3S1358	14, 18
D21S11	28, 31
D18S51	13
pentaE	11, 14
pentaD	9, 12
D8S1179	13
FGA	21

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cell culture flask

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Delivery expected until 9/25/2025

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