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HNO97 growing culture

HNO97 growing culture

Item no.	CLS-330129
Manufacturer	CLS Cell Lines Service
Amount	1 cell culture flask
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	Human (Homo sapiens)
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	HNO 97
Available
Tissue	Head-neck
Growth properties	Monolayer, adherent
Disease	Squamous cell carcinoma
Age	72 years
Gender	Male
Ethnicity	Caucasian
Morphology	Epithelial-like
Biosafety Level	1
Culture Medium	DMEM
Medium Supplements	10% FBS, 2 mM L-glutamine, 4.5 g/L glucose
Subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 5 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
Fluid Renewal	2 - 3 times per week
Freeze Medium	CM-1 (CLS catalog number 800150)
Amelogenin	X, X
Split Ratio	An initial ratio of 1:3 is recommended according to the growth rate
Manufacturer - Citation	HNO97 (CLS catalog number 300129)
CSF1PO	11
D13S317	12
D16S539	11
D5S818	11
D7S820	11, 13
TH01	9
TPOX	8
vWA	15, 19
D3S1358	14, 17
D21S11	28, 32.2
D18S51	22
pentaE	7, 11
pentaD	12, 13
D8S1179	8, 14
FGA	25
D1S1656	12, 13
D6S1043	13, 18
D2S1338	19
D12S391	19, 19
D19S433	14

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cell culture flask

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Delivery expected until 8/21/2025

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