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Panc-1 growing culture

Panc-1 growing culture

Item no.	CLS-330228
Manufacturer	CLS Cell Lines Service
Amount	1 cell culture flask
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	other
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	PANC-1, PANC.1, Panc 1, PanC1, Panc1, PANC1, Panc-1-P
Available
Age	56 years
Gender	Male
Ethnicity	Caucasian
Biosafety Level	1
Culture Medium	DMEM
Medium Supplements	10% FBS, 2 mM L-glutamine, 4.5 g/L glucose
Subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
Fluid Renewal	2 - 3 times per week
Freezing Recovery	After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 48 hours.
Freeze Medium	CM-ACF (CLS catalog number 800650)
Amelogenin	X, X
Tumorigenic	Growth in soft agar; formation of progressively growing carcinomas in nude athymic mice.
Split Ratio	A ratio of 1:2 to 1:4 is recommended
Seeding Density	1 x 10^4 cells/cm^2
Mutational Profile	Panc-1 cells carry a heterozygous Kras mutation in codon12: GGT(Wt Gly) > GAT(Asp)
Protein Expression	p53 positive, CEA negative
Karyotype	Three distinct marker chromosomes and one 1 ring chromosome
Isoenzymes	G6PD, B
Manufacturer - Citation	Panc-1 (CLS catalog number 300228)
CSF1PO	10, 12
D13S317	11
D16S539	11
D5S818	11, 13
D7S820	8, 1
TH01	7, 8
TPOX	8, 11
vWA	15
D3S1358	17
D21S11	28
D18S51	12
D8S1179	14, 15
FGA	21
D1S1656	12, 14
D2S1338	23, 24
D12S391	22
D19S433	11, 16
DQB1*	0, 0423728009259259
E	0, 0423728009259259

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cell culture flask

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Delivery expected until 9/25/2025

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