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RT-112 growing culture

RT-112 growing culture

Item no.	CLS-330324
Manufacturer	CLS Cell Lines Service
Amount	1 cell culture flask
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	other
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	RT 112, RT112
Available
Growth properties	Adherent
Age	Unspecified
Gender	Female
Ethnicity	Caucasian
Morphology	Epithelial-like
Biosafety Level	1
Culture Medium	RPMI 1640
Medium Supplements	10% FBS, 2 mM L-glutamine
Subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
Fluid Renewal	2 - 3 times per week
Freezing Recovery	About 50% viable cells, which recover within 24-48 hours
Freeze Medium	CM-ACF (CLS catalog number 800650)
Amelogenin	X, Y
Split Ratio	A ratio of 1:4 to 1:8 is recommended
Seeding Density	Start culture at 2-3 x 10^4 cells/cm^2 and continue with a seeding density of 1 x 10^4 cells/cm^2.
Protein Expression	p53 positive; Cytokeratine (4), 5, (6), 7, 8, 13, 17, 18, 19, Desmoplakin
Msi Status	Stable (MSS)
Isoenzymes	Yes, in nude mice
Manufacturer - Citation	RT-112 (CLS catalog number 300324)
CSF1PO	10, 11
D13S317	13, 14
D16S539	11, 13
D5S818	10, 13
D7S820	12, 11
TH01	7
TPOX	8, 11
vWA	14, 17
D3S1358	15
D21S11	27, 3
D18S51	15
pentaE	12, 16
pentaD	10, 11
D8S1179	13, 15
FGA	23

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cell culture flask

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Delivery expected until 8/21/2025

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