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WT-CLS1 growing culture

WT-CLS1 growing culture

Item no.	CLS-330379
Manufacturer	CLS Cell Lines Service
Amount	1 cell culture flask
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	other
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	CLS1
Available
Growth properties	Monolayer, adherent
Cell type	B lymphoblast
Age	5 years
Gender	Female
Ethnicity	Caucasian
Morphology	Epithelial-like
Biosafety Level	1
Viruses	HIV-1: negative, HBV: negative, HCV: negative
Culture Medium	IMDM
Medium Supplements	10% FBS, 2 mM L-glutamine
Subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
Fluid Renewal	Every 3 to 4 days
Freeze Medium	CM-ACF (CLS catalog number 800650)
Amelogenin	X, X
Tumorigenic	Yes, in nude mice; forms tumor with small cells consistent with Wilms' tumor (xenografts may not represent Wilmâ€™s tumors completely, see E. Kunce Stroup 2017)
Split Ratio	A ratio of 1:4 to 1:8 is recommended
Seeding Density	1-3 x 10^5 cells/cm^2
Mutational Profile	WT1 mutation status: wild type; CTNNB1 mutation status: wild type; no LOH.
Manufacturer - Citation	WT-CLS1 (CLS catalog number 300379)
CSF1PO	11, 13
D13S317	9, 11
D16S539	9, 11
D5S818	11, 12
D7S820	8, 1
TH01	9, 9.3
TPOX	8
vWA	15, 19
D3S1358	14, 19
D21S11	30, 31.2
D18S51	13, 15
pentaE	9, 12
pentaD	9
D8S1179	13, 14
FGA	22, 25

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cell culture flask

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Delivery expected until 8/28/2025

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