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HEp-2 growing culture

HEp-2 growing culture

Manufacturer	CLS Cell Lines Service
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	Human
Amount	1 cell culture flask
Item no.	CLS-330397
eClass 6.1	32160890
eClass 9.0	32160890
Available
tissue	Head-neck
disease	Adenocarcinoma
age	30 years
gender	Female
ethnicity	African American
morphology	Epithelial-like
growthProperties	Monolayer, adherent
citation	HEp-2 (CLS catalog number 300397)
biosafetyLevel	1
isoenzymes	G6PD, A
reverseTranscriptase	Negative
products	Keratin
cultureMedium	EMEM
mediumSupplements	10% FBS, 1 mM sodium pyruvate, 1% NEAA
subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
splitRatio	A ratio of 1:4 to 1:10 is recommended
seedingDensity	1 x 10^4 cells/cm^2
fluidRenewal	2 - 3 times per week
freezingRecovery	After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
freezeMedium	CM-ACF (CLS catalog number 800650)
amelogenin	X, X
CSF1PO	9, 1
D13S317	12, 13.3
D16S539	9, 1
D5S818	11, 12
D7S820	8, 12
TH01	7
TPOX	8, 12
vWA	16, 18
D3S1358	15, 18
D21S11	27, 28
D18S51	16
pentaE	7, 17
pentaD	8, 15
D8S1179	12, 13
FGA	18, 21

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cell culture flask

available

Delivery expected until 5/23/2024