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SW-620 growing culture

SW-620 growing culture

Manufacturer	CLS Cell Lines Service
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	other
Amount	1 cell culture flask
Item no.	CLS-330466
eClass 6.1	32160890
eClass 9.0	32160890
Available
metastaticSite	Lymph node
age	51 years
gender	Male
ethnicity	Caucasian
morphology	Epithelial-like
growthProperties	Adherent
citation	SW-620 (CLS catalog number 300466)
biosafetyLevel	1
tumorigenic	Yes, in athymic nude mice
karyotype	Average number of chromosomes 48 (range, 46-52). Eighteen marker chromosomes. For a detailed description of the karyotype we refer to Melcher et al.
cultureMedium	DMEM
mediumSupplements	10% FBS, 2 mM L-glutamine, 4.5 g/L glucose
subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
splitRatio	A ratio of 1:3 is recommended
fluidRenewal	2 times per week
freezeMedium	CM-1 (CLS catalog number 800150)
amelogenin	X, X
CSF1PO	13, 14
D13S317	12
D16S539	9, 13
D5S818	13
D7S820	8, 9
TH01	8
TPOX	11
vWA	16
D3S1358	16
D21S11	30, 30.2
D18S51	13
pentaE	10
pentaD	9, 15
D8S1179	13
FGA	24
D1S1656	13, 14
D6S1043	11, 12
D2S1338	17, 24
D12S391	17
D19S433	13

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cell culture flask

available

Delivery expected until 5/16/2024