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HROC313Met growing culture

HROC313Met growing culture

Item no.	CLS-330849
Manufacturer	CLS Cell Lines Service
Amount	1 cell culture flask
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	other
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	HROC313Met1
Available
Growth properties	Adherent
Age	72 years
Gender	Male
Ethnicity	Caucasian
Morphology	Epithelial-like
Biosafety Level	1
Viruses	Free of human pathogenic viruses SV40, JC/BK, HBV, HCV, HIV.
Culture Medium	DMEM:Ham' s F12
Medium Supplements	10% FBS, 2 mM L-glutamine
Subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add TrypLE Express (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37 Â°C for 10 to 15 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. This cell line will result in single cell suspension.
Fluid Renewal	Every 3 to 5 days
Freezing Recovery	5-7 days
Freeze Medium	CM-ACF (CLS catalog number 800650)
Tumorigenic	Yes, in immune-suppressed nude mice
Split Ratio	A ratio of 1:3 to 1:6 is recommended
Seeding Density	2 x 10^4 cells/cm^2
Metastatic Site	Lung; Metastasis of Primary CRC tissue
Doubling Time	52 hours
Mutational Profile	K-Rasmut, N-Raswt, H-Raswt, PIK3CAwt, B-Rafwt
Protein Expression	PTEN
Msi Status	MSS
Manufacturer - Citation	HROC313Met (CLS catalog number 300849)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cell culture flask

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Delivery expected until 9/18/2025

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