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P3X63Ag8.653 growing culture

P3X63Ag8.653 growing culture

Item no.	CLS-440118
Manufacturer	CLS Cell Lines Service
Amount	1 cell culture flask
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	Mouse (Murine, Mus musculus)
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	P3-X63-Ag8.653, P3-X63-Ag8-653, P3-x63-Ag8 653, P3-X63-Ag 8.653, P3-X63Ag8.653, P3-X63.Ag8.653, P3/X63/Ag8.653, P3X63 Ag8.653, P3X63 AG8-653, P3X63-Ag8.653, P3x63-Ag8.653, P3X63 AG 8.653, P3X63Ag8653, P3-X63-Ag8-6-5-3, P3X63Ag8-6-5-3, P3.times.63 Ag8.653, P3.653, X63-Ag 8.6.5.3, X63-AG 8.653, X63-Ag8-653, X63-Ag8.653, X63.Ag8.653, X63Ag8-653, X63Ag8.653, X63AG8.653, P3-653, GM03570, GM3570, GM03570E, NS653
Available
Tissue	Blood
Growth properties	Adherent/suspension
Disease	Myeloma
Gender	Female
Breed	BALB/c
Morphology	Round cells
Biosafety Level	1
Viruses	Tested negative for ectromelia virus (mouse pox).
Culture Medium	RPMI 1640
Medium Supplements	10% FBS, 2 mM L-glutamine
Subculturing	Subculture by collecting any floating cells in a centrifuge tube. Rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add TrypleExpress (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37 Â°C for 10 minutes. Combine all cells, and start new cultures at 4 x 10^5 cells/ml. The cell density should not exceed 2 x 10^6 cells/ml.
Fluid Renewal	Every 3 to 4 days; collect floating cells, centrifuge and add to the flask together with fresh medium.
Freezing Recovery	After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 48 hours.
Freeze Medium	CM-1 (CLS catalog number 800150)
Amelogenin	X, X
Seeding Density	1 x 10^4 cells/cm^2
Manufacturer - Citation	P3X63Ag8.653 (CLS catalog number 400118)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cell culture flask

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Delivery expected until 8/21/2025

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