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M2-10B4

M2-10B4

Item no.	CLS-440428
Manufacturer	CLS Cell Lines Service
Amount	1 cryovial
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	other
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	M210B4
Available
Growth properties	Adherent
Cell type	Fibroblast
Age	Unspecified
Gender	Female
Breed	C57BL/6J x C3H/HeJ
Morphology	Fibroblast-like
Biosafety Level	1
Culture Medium	RPMI 1640
Medium Supplements	10% FBS, 2 mM L-glutamine
Subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 5 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
Fluid Renewal	2 - 3 times per week
Freezing Recovery	Viability may be low after thawing.
Freeze Medium	CM-1 (CLS catalog number 800150)
Split Ratio	A ratio of 1:4 to 1:6 is recommended
Seeding Density	1 x 10^4 cells/cm^2
Products	Laminin; collagen IV (Collagen I(-), Factor VIII(-).
Manufacturer - Citation	M2-10B4 (CLS catalog number 400428)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cryovial

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Delivery expected until 9/4/2025

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